Type Of Client |
Nature of Work |
Number of Services |
Earning In Lakhs (Rs) |
Industries |
Preservative Efficacy of Liquid Orals / Anti Microbial / Anti HSV1 activity |
05 |
2.60 |
Educational Institutions |
In vitri high through put screening models on various diseases / Cytotoxicity Studies ? Microbiology Studies |
25 |
8.53 |
CONSULTANCY SERVICES
The department takes up the contract research services in the following areas for the benefit of the industrialists, academicians and students.
1. In vitro studies
- a. In vitro cytotoxicity studies on cell lines
- Determination of mitochondrial synthesis and cell viability by MTT assay.
- Determination of total cell protein content by Sulphorhodamine B (SRB) assay.
- Tryphan blue dye exclusion assay
- b. Anti-cancer studies
- Long term survival studies by clonolgenic assay
- Short term toxicity studies using Dalton’s Lymphoma Ascities (DLA) cells
- Apoptosis
- DNA Fragmentation Assay
- Nuclear Damage Assay
- c. Anti-adipogenic studies
- Oil red O staining method by using 3T3-L1 cell line.
- d. Hepatoprotective studies
- Primary cell culture (with the use of freshly isolated rat hepatocytes) and estimation of bio-chemical parameters (ASAT, ALAT, ALP, total protein, triglyceride, albumin, total bilirubin etc.).
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- On HEp-G2 Cell Lines
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- e. Anti-diabetic studies
- Glucose uptake assay by L-6 cell line as well as isolated rat hemi-diaphragm by using Glucose oxidase –Peroxidase (GOD-POD) Method.
- In vitro a-glucosidase inhibitory activity
- f. Anti-viral studies (HSV-I and HSV-II)
- Cytopathic Effect (CPE) inhibition assay
- Virucidal assay
- MTT antiviral assay
- Plaque reduction assay
- Time of addition studies
- g. Genotoxicity studies
- h. Histocompatibility and hematocompatibility of Nanoparticals as per ISO guidelines.
- i. In vitro wound healing assay
- Cell migration assay
- Wound dependent and independent Scratch assay
- j. In vitro burn assay on HaCaT cell line
2. Antioxidant studies
- Diphenyl Picryl Hydrazyl (DPPH) Radical Scavenging Method
- Scavenging Of Hydrogen Peroxide Radicals
- Nitric oxide radical inhibition activity
- Hydroxyl Radical Scavenging Activity by p-NDA (p-nitrosodimethyl aniline) method
- ABTS radical Scavenging method
- Scavenging of Superoxide radical by alkaline DMSO method
- Scavenging of hydroxyl radical by deoxyribose method
- Lipid peroxidation (LPO) assay
- Estimation of total antioxidant capacity by Phosphomolybdenum method
3. Antimicrobial Studies
- Estimation of Microbial load
- Agar absorption/diffusion method (Well and disc)
- Bio-autography method (direct and indirect)
- Minimum inhibitory concentration (MIC) determination.
- Stability studies
- Microbial examination of Soil.
4. Molecular Biology
- Isolation and estimation of DNA
- Isolation and estimation of RNA
- Protein isolation and estimation
- Western blotting assay
- Gene expression analysis by Real time PCR
5. Sterility testing as per standard protocol
- Solid dosage forms
- Liquid orals
- Medical devices
6. Hands on training programme offered by the Department for Industrialists/Academicians/Students
- Animal cell culture technology
- Microbiology
- Molecular biology