Who can enroll:
Post Graduate Students, Research scholars, Faculty member
Need/ Purpose:
To have Knowledge and hands-on experience of the basic Biochemical experiments required for Research purposes
Expected Outcome:
1. Having a thorough knowledge and implications of basic biochemical experiments required in Research
2.To be able to carry out the basic biochemical Experiments for their research study.
Learning objectives of the course
To provide hands-on training to the post graduate students, Research Scholars, Faculty of JSS Dental College in various biochemical methods that are essential in executing various research studies
Content/ syllabus:
The proposed value-added course is a 5-days long program involving theory as well as hands-on training sessions
Teaching & Learning activities:
1.Lectures
2.Demonstration
3.Hands On
4.Assessment
Resources:
1. Dr. Suman Basavaraju. Professor, Dept. of Periodontology
2. Dr Subbarao. and team Head, Dept. of Biochemistry
Assessment method:
Pre and Post Assessment with MCQs 60 for 30 minutes
Schedule:
Day-1:
Pre-assessment test (60 MCQs – 30minutes)
Theory (2h): pH – Definition, pH scale, pH meter, buffers, and functions of a buffer
Molarity, Molality, Equivalent weight, Normality, Inter-conversion of units etc.
Hands-on-training (4h): Calibration of pH meter, adjustment of pH using Buffers (acetate buffer and phosphate buffer), Preparation of Buffers, Indicator solutions
Day-2
Theory (1h): Principles of spectrophotometry and ELISA
Hands-on-training (5h): Absorption spectrum of proteins, nucleic acids, determination of 260 to 280 ratio, preparation of a standard graph, colorimetric and spectrophotometric quantification of phenolic compounds and proteins, estimation of salivary cytokines by ELISA
Day-3
Theory (1h): Principles of chromatography
Hands-on-training (5h): Separation of amino acids and sugars by paper chromatography, Size-exclusion chromatography of salivary proteins, HPLC of phenolic compounds
Day-4
Theory (1h): Electrophoresis – Principles and applications
Hands-on-training (5h): Preparation of agarose gels and polyacrylamide gels, Analysis of DNA by agarose gel electrophoresis, Analysis of proteins by PAGE followed by visualization of proteins by coomassie blue staining
Day-5
Theory (1h): PCR
Hands-on-Training (5h): Isolation of DNA by phenol-chloroform method; Amplification of target DNA by PCR
Day-6
Post assessment test (60MCQs; 30 minutes)
