DNA Barcoding is an upcoming tool used to identify or “barcode” the organism to make a distinction from other species for specimen identification and species discovery. To enrich our knowledge on DNA Barcoding tool, a Virtual Workshop on “DNA Barcoding” was organized for faculties, research scholars and postgraduate students on 24th and 25th of August 2020 by Department of Microbiology, School of Life Sciences, JSS Academy of Higher Education & Research, Mysuru.