CADRAT
Centre for Advanced Drug Research and Testing (CADRAT)
The Center for Advanced Drug Research and Testing (CADRAT), under the aegis of JSS Consultancy Trust, is functioning in the college to make effective use of the expertise and facilities available with the institution to the Government, public and private bodies in pursuit of advancement of pharmaceutical sciences and research.
Approvals:
The CADRAT has been approved by the Drugs Controller General of India, Government of India for carrying out bioavailability and bioequivalence of new drugs.
CADRAT is accredited by ISO 9001-2015 for drug product development by TUV-SUD.
Bioequivalence / Bioavailability studies
- The functions of BA/BE division
- Literature search and preparation of product profile.
- Method development
- Method validation protocol
- Method validation
- Audit
- Method SOP
- Analysis of clinical sample
- Repeated analysis
- Pharmacokinetics
- Statistical analysis
- Report
- Audit
- Final report
- Generation of chromatograms
Also the CADRAT takes up the contract research services in the following areas for the benefit of the industrialists, academicians and students.
Biological Testing
1. In vitro Screening studies
a. In vitro cytotoxicity studies on cell lines
- Determination of mitochondrial synthesis and cell viability by MTT assay.
- Determination of total cell protein content by Sulphorhodamine B (SRB) assay.
- Tryphan blue dye exclusion assay
b. Anti-cancer studies
- Long term survival studies by clonolgenic assay
- Short term toxicity studies using Dalton’s Lymphoma Ascities (DLA) cells
- Apoptosis
- DNA Fragmentation Assay
- Nuclear Damage Assay
c. Anti-adipogenic studies
- Oil red O staining method by using 3T3-L1 cell line.
d. Hepatoprotective studies
- Primary cell culture (with the use of freshly isolated rat hepatocytes) and estimation of bio-chemical parameters (ASAT, ALAT, ALP, total protein, triglyceride, albumin, total bilirubin etc.).
- On HEp-G2 Cell Lines
e. Anti-diabetic studies Glucose uptake assay by L-6 cell line as well as isolated rat hemi-diaphragm by using
- Glucose oxidase –Peroxidase (GOD-POD) Method.
- In vitro a-glucosidase inhibitory activity
f. Anti-viral studies (HSV-I and HSV-II)
- Cytopathic Effect (CPE) inhibition assay
- Virucidal assay
- MTT antiviral assay
- Plaque reduction assay
- Time of addition studies
g. Genotoxicity studies
h. Histocompatibility and hematocompatibility of Nanoparticals as per ISO guidelines. i. In vitro wound healing assay
- Cell migration assay
- Wound dependent and independent Scratch assay
j. In vitro burn assay on HaCaT cell line
2. Antioxidant studies
- Diphenyl Picryl Hydrazyl (DPPH) Radical Scavenging Method
- Scavenging Of Hydrogen Peroxide Radicals
- Nitric oxide radical inhibition activity
- Hydroxyl Radical Scavenging Activity by p-NDA (p-nitrosodimethyl aniline) method
- ABTS radical Scavenging method
- Scavenging of Superoxide radical by alkaline DMSO method
- Scavenging of hydroxyl radical by deoxyribose method
- Lipid peroxidation (LPO) assay
- Estimation of total antioxidant capacity by Phosphomolybdenum method
3. Antimicrobial Studies (Antibacterial and Antifungal studies)
- Agar absorption/diffusion method (Well and disc)
- Bio-autography method (direct and indirect)
- Minimum inhibitory concentration (MIC) determination.
4. Molecular Biology
- Isolation and estimation of DNA
- Isolation and estimation of RNA
- Protein isolation and estimation
- Western blotting assay
- Gene expression analysis by Real time PCR
5. Sterility testing as per standard protocol
- Solid dosage forms
- Liquid orals
- Medical devices
Drug Developments
- Preformulation studies
- Conventional drug delivery systems
- Novel drug delivery systems
- Herbal formulations
- Dissolution method developments
- Stability studies
- Dossier preparations
Preclinical Testing
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Toxicological studies
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Systemic Toxicity Studies
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Acute oral/dermal/IV/SC/IM toxicity studies
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Repeated dose oral/dermal/IV/SC/IM toxicity studies
Local Toxicity Studies:
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Acute Eye Irritation/Corrosion studies
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Acute Skin Irritation/Corrosion studies
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Skin Sensitization studies
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Carcinogenicity studies
-
Genotoxicity studies
Pharmacological Studies
- Antidiabetic studies: In vivo alpha glucosidase inhibition studies (Starch tolerance test); OGTT test; STZ model; Alloxan model, GTG model, fructose model, etc.
- Anti-obesity studies: Diet induced obesity models: Cafeteria diet-induced obesity, High-fat diet-induced obesity
- Anticancer studies: Dalton`s lymphoma ascites model; Solid tumor models
- Hepatoprotective studies: Paracetamol, carbon tetrachloride, Galactosamine models, etc.
- Antiulcer studies: Drug induced models, pyloric ligation models, stress induced models
- Aphrodisiac studies
- Analgesic studies: Eddy’s hot plate, Randal’s Sillito, tail flick, Incapacitance Tester, etc.
- Anti-inflammatory studies: Paw edema (Plethysmography)method, ear edema models, cotton-pellet model
- Hormone activity: Ovariectomized models, BPH models, etc.
- CNS stimulant/depressant/anxiety/depression models
- Learning and memory models
- Psoriasis studies: Mouse tail model
- Wound healing studies
- CVS models: antihypertensive models, Langendorff heart preparation, BP, ECG measurements using Labchart-7, AD Instruments
- Neurodegenerative models: Alzheimer’s models, antiparkinson’s models, etc.
Bioassays
Bioassay of various agonists/antagonists using isolated tissue preparations such as fundus strip, colon, ileum, jejunum, esophagus, vas deferens, anococcygeus muscle preparation, etc.
Computer Aided Drug Design
- Docking studies
- Pharmacophore based screening
- ADMET analysis
Analytical testing
- Development and validation of analytical/bioanalytical methods for drugs and pharmaceuticals/herbal products/food products by HPLC/LCMS/LCMS-MS
- Performance of stress degradation studies for the drugs and pharmaceuticals
- HPLC/LCMS/LCMS-MS
- Monograph analysis of pharmaceutical formulations/herbal formulations.
Director & CEO
Dr. S P Dhanapal Principal
JSS College of Pharmacy, Ooty
For Business:
Dr. K Gowthamarajan
Dy. Director
JSS College of Pharmacy, Ooty
Email:
gowthamsang@jssuni.edu.in
Mobile: 09443089812